Temperature. For the production of offspring, one female and two to three males are selected, which are kept for several hours in a spawning aquarium. As a rule, this is done in the evening. The temperature of tank system was generally maintained at 28 ± 0.5 °C using the submersible heater (55W) and the lighting condition are 14:10 hours (Westerfield, 2007). pH Level. Breeding protocols and husbandry details can be found in SI Appendix, Materials and Methods. The AB zebrafish strain and the Tg(vasa:vasa-egfp) zebrafish line of AB strain were used for the temperature treatment experiments. The original zebrafish (or zebra danio, Danio rerio) is a native of rivers in India and Bangladesh.It measures three centimeters long and has gold and dark blue stripes. Temperature: The document states on p. 24, “A water temperature of 28.5º C is widely cited as the optimum temperature for breeding zebrafish. The pH level of the water should be 6-8. Zebrafish Breeding. ... Zebrafish initiate breeding at the onset of white light. Fertilized egg s can be obtained either through in-tank breeding or pairwise breeding. During fertilization, the female begins to spawn in the morning. If the water temperature is maintained then the fish can live comfortably without any health issues, so try to check the temperature while setting water in the fish tank. Breeding of zebrafish in the laboratory environment for resea rch . The temperature of the water in the aquarium of Zebra Danio should be 64-74° F (18-24° C). Zebrafish are native to South Asia where they are found in streams, ponds and other slow-flowing water bodies including rice fields. deformities when cold developmental temperatures in zebrafish offspring mismatched warm temperatures experienced by parents. DNMT3a knock-out genotype interacted with Hardness development . Thanks to the constant climate chamber’s LED lighting active from 8 am to 10 pm, After breeding zebrafish with the highest levels of thermal tolerance for six generations, we found that this upper limit increased by 0.04°C with each new generation. Especially relevant is air purging with increasing water temperature. There has however, been little research to investigate the full implications of constantly keeping fish at this very specific temperature.” Temperature control in sub-optimal settings is also ex ceptionally important because of its profound effects . Interestingly, short-term cold acclimation of parents before breeding rescued DNMT3a knock-out offspring by restoring survival at cold temperatures. Zebrafish are photoperiodic in their breeding, and produce embryos every morning, shortly after sunrise. 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