endobj << 128 0 obj /D [19 0 R /FitR 52 495 290 495] The information gathered in this review collectively indicates the zebrafish embryotoxicity model holds a great promise in herbal toxicity assessment and provides an ideal alternative to laboratory animals like rats, mice, or rabbits. PLoS ONE (2012-01-01) . /D [19 0 R /FitR 52 514 290 514] 2- Place the embryos at the expected developmental stage into a pre-chilled dounce glass and add 1 ml of ice-cold MB buffer. << The zebrafish model is a powerful tool for studying developmental and apoptotic processes in vertebrates. << To facilitate the application of … /EmbeddedFiles 8 0 R /D [28 0 R /FitR 52 702 290 702] The protocol detailed in this work describes the application of phage therapy against Pseudomonas aeruginosa infection in CF zebrafish embryos. /LW 1 131 0 obj >> A common approach to unravel the gene networks that drive biology is to examine the consequences of manipulating genes. /D [29 0 R /FitR 52 124 290 124] endobj >> (CR80) 109 0 R (CR81) 110 0 R (CR82) 111 0 R (CR83) 112 0 R (CR84) 113 0 R /Parent 10 0 R << << >> /TrimBox [0 -5.66927 595.276001 785.197021] /MediaBox [0 -5.66927 595.276001 785.197021] 160 0 obj >> >> 24 0 obj 46 0 obj >> 94 0 obj << Acrobat Distiller 6.0 (Windows) /Rotate 0 << << 135 0 obj /D [21 0 R /FitR 307 682 545 682] The gain, saturation, exposure, and lighting levels were identical for each imaging procedure. >> << << endobj Zebrafish embryos were kept in a 28 °C incubator in E3 media (5 mM NaCl, 0.33 mM MgSO 4, 0.33 mM CaCl 2, 0.17 mM KCl, and 0.1% methylene blue). >> Fluorescence techniques used to probe biomolecular dynamics and interactions of molecules in live zebrafish embryos are therefore highly sought … /D [28 0 R /FitR 52 513 290 513] By manipulating the local temperature within an embryo, spatial control of transgene expression is also possible. 1- Collect embryos at the one-cell stage in a 35 mm Petri dish with E3 medium (100 embryos per condition at least). Science. >> 202 0 R] << 156 0 obj /ca 1 Figure 2. �[2{��o �O}�����m�glۣ�M�% 8�X�����^h?\mm ��&*���Dj��o]fGJy}�֥����W.�� USA.gov. /StructParents 0 AU - Zhu, Xiaoshan. -, Huisken J, Swoger J, Del Bene F, Wittbrodt J, Stelzer EHK. >> 40 0 obj << 65 0 obj 159 0 obj /Annots [403 0 R] induce lesions in zebrafish embryos. 2004;305:1007–1009. ZebraBeat: a flexible platform for the analysis of the cardiac rate in zebrafish embryos… Zebrafish eggs are fertilized and develop outside of the mother's body making visualization with high resolution imaging easier for researchers. endobj endobj 2003 Aug 29;225(2):177-82. doi: 10.1016/S0378-1097(03)00446-4. << Nature. 13 0 obj 102 0 obj /Type /Metadata The zebrafish (Danio rerio) is a freshwater fish belonging to the minnow family of the order Cypriniformes.Native to South Asia, it is a popular aquarium fish, frequently sold under the trade name zebra danio (and thus often called a "tropical fish" although both tropical and subtropical).. >> endobj Much of the early modern work using this embryo model began with the papers of Kimmel.Several large laboratories in the US are now developing large breeding programs to carry out \"k… << 109 0 obj endobj /Last 9 0 R >> 63 0 obj << /MediaBox [0 0 594.95996 840.95996] /Annots [407 0 R 408 0 R 409 0 R] >> /rgid (PB:5280249_AS:230847370493954@1432049710276) endobj >> 45 0 obj 33 0 obj The results showed disruption in genes associated with muscle development, … endobj << >> Zebrafish larvae up to 8 days of post fertilization (dpf) are suitable for high throughput screening because they can grow without nutrient supply. 11 0 obj endobj 175 0 obj 10 0 obj >> /D [27 0 R /FitR 307 442 545 442] << endobj /ExtGState << Tg(ola.sp7:nlsGFP) transgenic zebrafish was imaged with a laser scanning confocal microscope (LSCM) (Leica TCS SP5 II, Germany) for 3D reconstruc-tion. << endobj /Dest (Section11) endobj /Type /Page /D [27 0 R /FitR 307 622 545 622] /D [29 0 R /FitR 52 652 290 652] 23 0 obj 1 0 obj ... Vitrification of zebrafish embryo blastomeres in microvolumes. Hence, zebrafish have been recognized as a valuable model species for epigenetics. 117 0 obj endobj >> 151 0 obj correction of cell membrane movement during measurement and correction of spectral cross-talk of fluorescent proteins. >> >> >> 71 0 obj 171 0 obj /Dest (Section16) 2. Zebrafish and humans share 70% of the same genes. 149 0 obj /Rotate 0 /CropBox [0 -5.66927 595.276001 785.197021] 89 0 obj Anom. endobj Caged … 93 0 obj << >> /Thumb 377 0 R << << endobj endobj endobj We found that the majority of zinc-finger proteins assembled from these modules have favorable specificities and nearly one-third of modular ZFNs generated lesions at their targets in the zebrafish genome. Drug Discov. /D [27 0 R /FitR 307 142 545 142] >> 83 0 obj Work More Efficiently in Developmental Biology With Stereo Microscopy: Zebrafish, Medaka, and Xenopus. /Count 12 6. cRSI of living zebrafish embryo wound response. endobj 140 0 obj AU - Zhu, Xiaoshan. 26 0 obj (Section12) 154 0 R (Section13) 155 0 R (Section14) 156 0 R (Section15) 157 0 R] Methods Mol. /Names [(CR67) 94 0 R (CR68) 95 0 R (CR69) 96 0 R (CR7) 97 0 R (CR70) 98 0 R /D [27 0 R /FitR 307 642 545 642] /MediaBox [0 -5.66927 595.276001 785.197021] /Rotate 0 endobj endobj 127 0 obj 2012 Mar 15;(61):3781. doi: 10.3791/3781. endobj /Title << Mating and egg harvesting procedure for zebrafish: 1. >> 134 0 obj << 122 0 obj endobj endobj /Parent 11 0 R /Limits [(CR1) (CR66)] /TrimBox [0 -5.66927 595.276001 785.197021] /Kids [18 0 R 19 0 R 20 0 R 21 0 R 22 0 R 23 0 R 24 0 R 25 0 R 26 0 R 27 0 R /D [19 0 R /FitR 52 421 290 421] The practical application of cryopreservation in the aquatic species needs more vigorous research efforts in this area and the efforts may be prioritized on endangered, economical value and representative species from various aquatic habitats. Phage Therapy Application to Counteract Pseudomonas aeruginosa Infection in Cystic Fibrosis Zebrafish Embryos Authors: Cafora M, Forti F, Briani F, Ghisotti D, Pistocchi A 148 0 obj AU - Wang, Jiangxin. endobj /D [29 0 R /FitR 307 224 545 224] /Names [(CR1) 30 0 R (CR10) 31 0 R (CR100) 32 0 R (CR11) 33 0 R (CR12) 34 0 R endobj endobj Evaluation of 14 organic solvents and carriers for screening applications in zebrafish embryos and larvae. << << << << << << >> << << /MediaBox [0 -5.66927 595.276001 785.197021] endobj >> 155 0 obj We then give an overview of new FCS modalities that are designed to cope with emerging challenges in intracellular applications, e.g. In recent years, the zebrafish embryo has increasingly attracted the attention of chemists and pharmacologists for its utility in identifying chemicals with pharmacological activity in a whole-animal context. << /D [20 0 R /FitR 307 221 545 221] /D [29 0 R /FitR 52 622 290 622] endobj endobj 67 0 obj /Rotate 0 >> /D [27 0 R /FitR 307 612 545 612] endobj 90 0 obj endobj /D [29 0 R /FitR 307 533 545 533] endobj << (HypSec4) 144 0 R (HypSec5) 145 0 R (HypSec6) 146 0 R (HypSec7) 147 0 R (HypSec8) 148 0 R >> Y1 - 2012. >> >> The method is suited for a wide range of applications including study of transgenic zebrafish of unlimited size, immunostaining of whole-mount embryos, cell tracking and on-section enzyme histochemistry of embryonic or even adult zebrafish. Zebrafish-Mycobacterium marinum model for mycobacterial pathogenesis. >> N2 - As an emerging model for toxicological studies, zebrafish has been explored for nanotoxicity assessment. /Count 11 endobj Tools for gene manipulation together with information about the genome are powerful resources for investigating any biological process. Automated Microscopy for Zebrafish And Other Whole Organism Screening Applications. /MediaBox [0 -5.66927 595.276001 785.197021] >> /D [28 0 R /FitR 307 732 545 732] 4 0 obj /D [28 0 R /FitR 307 293 545 293] Fig. correction of cell membrane movement during measurement and correction of spectral cross-talk of fluorescent proteins. endobj 36 0 obj endobj /D [19 0 R /FitR 52 334 290 334] endobj >> 99 0 obj endobj National Center for Biotechnology Information, Unable to load your collection due to an error, Unable to load your delegates due to an error, Confocal Raman spectroscopic imaging (cRSI) was used to image a fixed embryo (, Confocal Raman spectroscopic imaging (cRSI) was used to image tissue regions of interest at 3 days post fertilization, with a spatial resolution of 0.5–1 µm. /BitsPerComponent 8 /BM /Normal This need is generating increased interest in the use of zebrafish embryos as both a screening tool and an alternative to mammalian test methods. /D [22 0 R /FitR 0 786 596 0] A short description of the preparation of zebrafish embryos for FCS measurements is included. We report the synthesis, characterization, and in vivo application of light-activated, negatively charged peptide nucleic acids (caged ncPNAs) for down-regulating gene expression during zebrafish embryonic development. endobj 130 0 obj << << /Type /Filespec This review evaluates the potential application and suitability of the zebrafish model in the development of biomaterials and drug delivery systems by investigating the topic of exposure and evaluating test substances on zebrafish embryos and how the FET assay can be applied to evaluate biocompatibility. /Resources 281 0 R The zebrafish is an important and widely used vertebrate model organism in scientific … doi: 10.1038/nature11057. The accessibility of its externally fertilized embryos allows numerous manipulations, and the transparency of the zebrafish embryo permits direct serial visualization of biological processes in vivo (Beis and Stainier, 2006). /Parent 4 0 R 106 0 obj /Thumb 282 0 R (CR5) 75 0 R (CR50) 76 0 R (CR51) 77 0 R (CR52) 78 0 R (CR53) 79 0 R << << 62 0 obj >> Whole organisms offer several advantages over cell lines for forward chemical genetic screens, providing information … Zebrafish heg1 ∆25 embryos at 48 hpf were treated with each drug to determine the optimal concentration for recovery. /D [28 0 R /FitR 307 353 545 353] 3B2 Total Publishing System 8.07e/W Unicode << /MediaBox [0 -5.66927 595.276001 785.197021] /ProcSet [/PDF /Text /ImageB /ImageC /ImageI] >> /XObject << 76 0 obj >> /D [27 0 R /FitR 307 322 545 322] 66 0 obj 60 0 obj endobj endobj /D [29 0 R /FitR 52 244 290 244] �+Sl�V����˗���Gޗ"���%{O���ȇ�,Ej籬s�/�rF �}S��t���6�Z����;[�� From effects of nanosized HA, SiO(2) and TiO(2) particles on the zebrafish embryos development, they were adsorbed on the membrane surface confirmed by the electronic scanning microscopy. /Resources 339 0 R CryoLetters. Get the latest public health information from CDC: https://www.coronavirus.gov, Get the latest research information from NIH: https://www.nih.gov/coronavirus, Find NCBI SARS-CoV-2 literature, sequence, and clinical content: https://www.ncbi.nlm.nih.gov/sars-cov-2/. /MediaBox [0 -5.66927 595.276001 785.197021] >> (CR54) 80 0 R (CR55) 81 0 R (CR56) 82 0 R (CR57) 83 0 R (CR58) 84 0 R << << /TrimBox [0 -5.66927 595.276001 785.197021] << >> %PDF-1.3 endobj /Type /Page 72 0 obj 8 0 obj /MediaBox [0 -5.66927 595.276001 785.197021] endobj 44 0 obj >> Fig. (CR9) 119 0 R (CR90) 120 0 R (CR91) 121 0 R (CR92) 122 0 R (CR93) 123 0 R /Parent 10 0 R >> endobj << The attributes of the zebrafish have established it as a powerful model for the study of vertebrate development. /D [27 0 R /FitR 52 521 290 521] -. endobj 16 0 obj /D [27 0 R /FitR 307 182 545 182] /BM /Normal 17 0 obj /D [28 0 R /FitR 52 483 290 483] >> /Prev 16 0 R endobj /D [27 0 R /FitR 307 692 545 692] endobj This site needs JavaScript to work properly. /BM /Normal Despite these caveats, several features of zebrafish—including the available genetic resources and transparency of the embryo—have proven useful in a variety of research applications. 79 0 obj /D [29 0 R /FitR 307 333 545 333] High-resolution confocal Raman spectroscopic imaging for anatomical tissue characterization. Phage Therapy Application to Counteract Pseudomonas aeruginosa Infection in Cystic Fibrosis Zebrafish Embryos Authors: Cafora M, Forti F, Briani F, Ghisotti D, Pistocchi A /D [29 0 R /FitR 307 732 545 732] Low cost, easy handling, the requirement of small quantities of test compounds, and high t… /First 16 0 R Adult zebrafish, both male and female, were mixed and maintained at 28 °C with a 14/10 hour light/dark cycle. One such way for inducing heat shock response in targeted cells is by using a laser microbeam under the microscope. << stream endobj 1. << endobj /Parent 10 0 R >> /Type /Page endobj 81 0 obj This shows manual dechorionating (removing the shell) from 1 day old zebrafish embryos. /Rotate 0 << PY - 2012. x��yp��}�h i�f&i2�2S�4d�IҤM�I�v��1M�6�N2iC�M�dhJ��Ʒ�|��`cc|b�ԧ$�>�u�CƦHZ�m�p��:˫߻Z��~�Ϯ���}w�w?�~��_�R Ѕ7����������f���������R�:f���z����\i�t�*\�n��]߸�Kw�7�Q޺63�p�:r�d�����k�-�U����t���!�z��1�l�k�k��͍h�mlm=�N���.l��6�k��j���ce�p��p�� ? endobj Recently we showed the critical role of two members of the Bcl-2 family of proteins during early zebrafish development. endobj >> >> /D [20 0 R /FitR 52 271 290 271] /SA true With this technique, parental transfer of lipophilic contaminants may be mimicked. /Contents 203 0 R /Rotate 0 Biomolecular profiling and discrimination of…. uuid:fbae0592-01de-4f93-afdb-94400ed6fd65 /D [29 0 R /FitR 52 423 290 423] 124 0 obj >> 58 0 obj << /ca 1 Zebrafish embryos provide a unique opportunity to visualize complex biological processes, yet conventional imaging modalities are unable to access intricate biomolecular information without compromising the integrity of the embryos. /Contents 241 0 R 6 0 obj 114 0 obj /D [29 0 R /FitR 307 373 545 373] 176 0 obj Zebrafish embryos were kept in a 28 °C incubator in E3 media (5 mM NaCl, 0.33 mM MgSO 4, 0.33 mM CaCl 2, 0.17 mM KCl, and 0.1% methylene blue). endobj ... we determined the maximum tolerated concentration (MTC) of several commonly used solvents and carriers in zebrafish embryos and larvae at various developmental stages. endobj 143 0 obj endobj Fig. 28 0 R] >> 170 0 obj << << 54 0 obj >> /TrimBox [0 -5.66927 595.276001 785.197021] /D [28 0 R /FitR 52 224 290 224] 59 0 obj /Prev 172 0 R >> >> /Height 320 /D [23 0 R /FitR 0 786 596 0] << /CA 1 144 0 obj Figure 2. >> << << endobj 80 0 obj 4. 88 0 obj 42 0 obj /Pages 5 0 R These embryos have the great advantage that they develop as \"see through\" embryos, that is, all internal development can be clearly observed from the outside in the living embryo. >> 5. /D [28 0 R /FitR 52 303 290 303] Fig. >> >> endobj << The thin endothelial wall of a newly formed vessel is under enormous stress at the onset of blood flow, rapidly acquiring support from mural cells (pericytes and vascular smooth muscle cells; vSMCs) during development. 141 0 obj << 2013;783:251-66. doi: 10.1007/978-1-4614-6111-1_13. << 113 0 obj 69 0 obj /Limits [(Section16) (maintitle)] << Adv Exp Med Biol. /D [27 0 R /FitR 52 102 290 102] /Rotate 0 << /Count 1 endobj /D [23 0 R /FitR 52 508 290 508] endobj /D [27 0 R /FitR 52 219 290 219] endobj << << << Commun.6, 7790 (2015). More recently, zebrafish-based assays have proven useful for structure-activity relationship studies [6]. << endobj endobj << endobj << Congenit. /D [28 0 R /FitR 52 264 290 264] >> De Luca, E. et al. /Contents 404 0 R /Type /Page /D [29 0 R /FitR 307 562 545 562] << >> Swaim LE, Connolly LE, Volkman HE, Humbert O, Born DE, Ramakrishnan L. Infect Immun. /D [29 0 R /FitR 307 303 545 303] /Resources 324 0 R /Type /Page << AU - Chang, Yung.  |  Position of 10x images relative to 1 dpf zebrafish embryo. 4. << >> << /Next 17 0 R >> 2018 Nov 1;150:32-41. doi: 10.1016/j.ymeth.2018.07.004. Hence, this review intends to provide a comprehensive account of available scientific evidence of the application of the zebrafish embryos to assess the herbal toxicity. << /Type /Page 108 0 obj endobj endobj 61 0 obj /D [28 0 R /FitR 52 572 290 572] >> /D [29 0 R /FitR 52 732 290 732] Univariate analysis was performed by integrating over a wavenumber range corresponding to relevant biomolecules: collagen-rich regions at 918 ± 20 cm, An established model was used in which zebrafish embryos were injected with. /Count 1 have been recognized as causative agents of life-threatening systemic infections, primarily in… Read article Nov 25, 2016 Abstract Stereo Microscopy. << /Filter /FlateDecode << endobj /D [28 0 R /FitR 52 194 290 194] Epub 2018 Jul 27. /ModDate (D:20141113140224+01'00') /Kids [12 0 R 13 0 R 14 0 R] /Thumb 413 0 R Zebrafish or zebra danio (danio rerio) are seen as one of the latest \"models\" for vertebrate embryological development studies. << /Thumb 325 0 R /D [28 0 R /FitR 52 144 290 144] /CropBox [0 -5.66927 595.276001 785.197021] 15 0 obj endobj Both dyes have shown potential for toxicity; however, there is no comprehensive understanding of their effects. 336 0 R 337 0 R] /D [19 0 R /FitR 307 383 545 383] endobj Optical sectioning deep inside live embryos by selective plane illumination microscopy. 150 0 obj << endobj << /SMask 419 0 R /D [28 0 R /FitR 307 493 545 493] 2006 Nov;74(11):6108-17. doi: 10.1128/IAI.00887-06. >> /CropBox [0 -5.66927 595.276001 785.197021] 118 0 obj /D [27 0 R /FitR 307 272 545 272] << 27 0 obj >> 53 0 obj endobj >> 55 0 obj >> endobj In particular, multiple zebrafish embryos can be exposed to waterborne test chemicals in a single well of a 96- or 384-well tissue culture plate. /D [19 0 R /FitR 307 321 545 321] << /Parent 10 0 R >> >> endobj << /CropBox [0 -5.66927 595.276001 785.197021] Volumetric confocal Raman spectroscopic imaging of whole zebrafish embryos. /First 170 0 R 234 0 R 235 0 R 236 0 R 237 0 R 238 0 R 239 0 R 240 0 R] << endobj 139 0 obj >> /D [28 0 R /FitR 307 194 545 194] << << endobj >> Here, we report the use of confocal Raman spectroscopic imaging for the visualization and multivariate analysis of biomolecular information extracted from unlabeled zebrafish embryos. /D [21 0 R /FitR 307 682 545 682] >> /Thumb 243 0 R >> << /D [29 0 R /FitR 307 702 545 702] endobj endobj /Resources 212 0 R /Contents [204 0 R 205 0 R 206 0 R 207 0 R 208 0 R 209 0 R 210 0 R 211 0 R] /D [19 0 R /FitR 52 334 290 334] << /Parent 5 0 R /D [27 0 R /FitR 307 372 545 372] >> /D [27 0 R /FitR 307 402 545 402] /Parent 5 0 R /CreationDate (D:20080715065123+08'00') << Cell microinjection is a technique of precise delivery of substances into cells and is widely used for studying cell transfection, signaling pathways, and organelle functions. endobj Would you like email updates of new search results? /D [19 0 R /FitR 307 383 545 383] Microinjection of the embryos of zebrafish, the third most important animal model, has become a very useful technique in bioscience. 169 0 obj >> More recently, the zebrafish embryo has been applied … /F6 180 0 R /D [27 0 R /FitR 307 492 545 492] The utility of fish embryos for pesticide hazard assessment was investigated by comparing published zebrafish embryo toxicity data from pesticides with median lethal concentration 50% (LC50) data for juveniles of 3 commonly tested fish species: rainbow trout, bluegill sunfish, and sheepshead minnow. /Annots [393 0 R 394 0 R 395 0 R 396 0 R 397 0 R 398 0 R 399 0 R] (CR71) 99 0 R (CR72) 100 0 R (CR73) 101 0 R (CR74) 102 0 R (CR75) 103 0 R /D [28 0 R /FitR 307 543 545 543] endobj << >> >> >> The ACQUIFER Imaging Machine is a versatile and flexible high content screening platform following a unique opto-mechanical design, optimized for non-adherent and motion-sensitive specimen. /D [21 0 R /FitR 52 171 290 171] /D [29 0 R /FitR 52 702 290 702] 172 0 obj /TrimBox [0 -5.66927 595.276001 785.197021] 153 0 obj /G3 173 0 R FEMS Microbiol Lett. endobj >> /D [29 0 R /FitR 307 493 545 493] /D [28 0 R /FitR 307 224 545 224] 104 0 obj 107 0 obj >> 86 0 obj >> endobj /Thumb 402 0 R /Type /Page /Contents 338 0 R /Resources 405 0 R /Parent 10 0 R >> 5. organization levels. /MediaBox [0 -5.66927 595.276001 785.197021] << (HypFig3) 139 0 R (HypFig4) 140 0 R (HypSec1) 141 0 R (HypSec2) 142 0 R (HypSec3) 143 0 R One of the first applications for the zebrafish embryo in environmental sciences was promoted by the aim to Fig. endobj Volumetric confocal Raman spectroscopic imaging…. /Parent 17 0 R 101 0 obj Spinning disk confocal imaging of neutrophil migration in Zebrafish. /TrimBox [0 -5.66927 595.276001 785.197021] >> /D [29 0 R /FitR 52 383 290 383] Schematic overview of the confocal Raman spectroscopic imaging applications in zebrafish embryos developed…, Fig. /Contents 280 0 R >> Zebrafish embryos provide a unique opportunity to visualize complex biological processes, yet conventional imaging modalities are unable to access intricate biomolecular information without compromising the integrity of the embryos. /D [28 0 R /FitR 52 104 290 104] /F 414 0 R >> (CR63) 90 0 R (CR64) 91 0 R (CR65) 92 0 R (CR66) 93 0 R] /Dest (Section17) 162 0 obj >> << << >> Nat. << (CR27) 50 0 R (CR28) 51 0 R (CR29) 52 0 R (CR3) 53 0 R (CR30) 54 0 R 91 0 obj << 31 0 obj >> >> Its experimental virtues make it an ideal system with which to identify new bioactive molecules, and to assess their toxicity and teratogenicity at medium-to-high throughput. << application/pdf Here, we report the use of confocal Raman spectroscopic imaging for the visualization and multivariate analysis of biomolecular information extracted from … << >> 3 (A), the use of the AL treatment has led to an apparently significant lethality rate of the early zebrafish embryos, but fortunately with development progresses of embryos, the death rate decreased. Position of 10x images relative to 1 dpf zebrafish embryo. (CR31) 55 0 R (CR32) 56 0 R (CR33) 57 0 R (CR34) 58 0 R (CR35) 59 0 R doi: 10.1080/17460441.2017.1297416. << endobj One reason for the success of zebrafish as a model organism is its amenability to genetic manipulation. In zebrafish embryos, downstream gene expression can be greatly induced throughout the body by raising the temperature from 28.5°C to 38.0°C. 100 0 obj Brown HK, Schiavone K, Tazzyman S, Heymann D, Chico TJA. /Type /Page endobj 47 0 obj >> 120 0 obj >> <<  |  << 2014-11-13T14:02:24+01:00 Infection of zebrafish embryos with intracellular bacterial pathogens. 2008-07-15T06:51:23+08:00 VEGF Inhibitor Treatment 84 0 obj << /D [28 0 R /FitR 307 264 545 264] >> /D [29 0 R /FitR 52 523 290 523] << >> A short description of the preparation of zebrafish embryos for FCS measurements is included. >> /D [20 0 R /FitR 52 271 290 271] << The optical transparency of zebrafish embryos and their amenability to genetic manipulation (e.g., gene knockdown or expression of fluorescent proteins in specific tissues/organs) are extremely advantageous to precisely track the release dynamics of loaded drugs and investigate the underlying mechanisms of nano–bio interactions, which can greatly facilitate the optimization of nanomedicine formulations and predict the efficacy of … During all the imaging processes of all the groups of fish, the << endobj endobj Time-lapse imaging beyond the diffraction limit. /D [25 0 R /FitR 307 546 545 546] /Parent 9 0 R /D [28 0 R /FitR 52 632 290 632] << endobj << See this image and copyright information in PMC. The utility of fish embryos for pesticide hazard assessment was investigated by comparing published zebrafish embryo toxicity data from pesticides with median lethal concentration 50% (LC50) data for juveniles of 3 commonly tested fish species: rainbow trout, bluegill sunfish, and sheepshead minnow. >> /ColorSpace /DeviceRGB Bcl-2 proteins can be localized in different cellular compartments including mitochondria and endoplasmic reticulum and their functional characterization often requires specific subcellular membrane fractionation. << In the area of toxicology, a number of in vivo assays based on zebrafish embryos and larvae are now available to examine the potential toxicity of compounds early in the drug discovery process [7,8], as AU - Chen, Yongsheng. /D [19 0 R /FitR 307 148 545 148] >> 2012;485:471–477. << /D [20 0 R /FitR 0 786 596 0] 3 0 obj 49 0 obj /Title /Contents 390 0 R (CR94) 124 0 R (CR95) 125 0 R (CR96) 126 0 R (CR97) 127 0 R (CR98) 128 0 R >> /Kids [15 0 R] 52 0 obj endobj 14 0 obj /D [29 0 R /FitR 307 602 545 602] >> endobj << /LJ 0 DNA methylation patterns are reprogrammed in pre-implantation zebrafish embryos, as in mammals . >> Early zebrafish embryos are less than 1 mm in diameter, allowing several embryos to fit easily in a single well of a 384-well plate. The application of a chemical dispersant increased the acute toxicity most likely due to a higher bioavailability of dissolved and … /D [28 0 R /FitR 307 712 545 712] 254 0 R 255 0 R 256 0 R 257 0 R 258 0 R 259 0 R 260 0 R 261 0 R 262 0 R 263 0 R 12 0 obj /Thumb 392 0 R endobj /Thumb 355 0 R endobj doi: 10.1007/978-1-62703-845-4_14. /D [28 0 R /FitR 52 672 290 672] /Resources 401 0 R >> /D [28 0 R /FitR 307 393 545 393] endobj /Thumb 213 0 R /D [20 0 R /FitR 307 246 545 246] >> 111 0 obj >> >> >> (CR13) 35 0 R (CR14) 36 0 R (CR15) 37 0 R (CR16) 38 0 R (CR17) 39 0 R /Type /Page /D [27 0 R /FitR 307 543 545 543] Confocal Raman spectroscopic imaging analysis…. /Thumb 340 0 R /F (folder.joboptions) 168 0 obj /Title uuid:4ba6ad0e-11ce-4e40-ae92-e950148537ca 2. m�k���N�� >> >> 138 0 obj Preparation of zebrafish embryos extracts. 115 0 obj << >> << endobj endobj /D [28 0 R /FitR 307 114 545 114] /D [29 0 R /FitR 52 453 290 453] << endobj • Protocols using zebrafish embryos allow for much greater throughput than traditional animal tests, making the embryonic zebrafish … << /Limits [(CR67) (Section15)] COVID-19 is an emerging, rapidly evolving situation. 133 0 obj /Count -6 >> >> /Type /Catalog /Type /Page >> >> /D [27 0 R /FitR 52 171 290 171] Mycobacterium marinum infection of adult zebrafish causes caseating granulomatous tuberculosis and is moderated by adaptive immunity. /Prev 417 0 R 32 0 obj 123 0 obj >> << HHS Expert Opin. 166 0 obj Here, we describe zebrafish-based assays, including both embryo and adult, for evaluation of nanotoxicity caused by metal oxide nanoparticles (NPs), in particular, zinc oxide (ZnO) and titanium oxide (TiO2) nanoparticles. /D [29 0 R /FitR 307 134 545 134] >> endobj endobj /Rotate 0 >> /Names 3 0 R Evaluation of 14 organic solvents and carriers for screening applications in zebrafish embryos and larvae PLoS One. endobj 1. %���� /Parent 17 0 R endobj endobj Fig. Biomolecular profiling and discrimination of mycobacterial infections. The SiO(2) particles were distributed throughout the outer surface … << 2012;7(10 ):e43850. << << Schematic overview of the confocal…, Fig. /Parent 10 0 R endobj 29 0 obj (Section211) 163 0 R (Section214) 164 0 R (Section312) 165 0 R (Section313) 166 0 R (maintitle) 167 0 R] >> << Application example of HyVolution Super-Resolution - Bacteria belonging to the genus Cronobacter spp. Early-life environmental exposure to toxic chemicals has a long-term influence on the development and health of living organisms. 25 0 obj 136 0 obj 87 0 obj endobj NLM >> << /Parent 10 0 R 224 0 R 225 0 R 226 0 R 227 0 R 228 0 R 229 0 R 230 0 R 231 0 R 232 0 R 233 0 R 105 0 obj /Font << Fig. Brain-wide neuronal dynamics during motor adaptation in zebrafish. << /PageMode /UseThumbs /Type /Pages << /D [29 0 R /FitR 52 553 290 553] /First 9 0 R /D [29 0 R /FitR 307 174 545 174] /Last 17 0 R << /X10 177 0 R >> 388 0 R 389 0 R] 37 0 obj 82 0 obj << 56, 18–27 (2016). 116 0 obj 34 0 obj /ca .2 /D [28 0 R /FitR 307 134 545 134] /Contents 353 0 R /Names [ 168 0 R] 92 0 obj endobj Benard EL, van der Sar AM, Ellett F, Lieschke GJ, Spaink HP, Meijer AH. Of proteins during early zebrafish development the aim to Fig of whole zebrafish embryos for FCS measurements included. 11 ):6108-17. doi: 10.1128/IAI.00887-06 infections, primarily in… Read article Nov 25, 2016 Abstract Microscopy. Trunk of the yolk sac so that the resulting image is set around trunk. Then give an overview of the Bcl-2 family of proteins during early development! Glass vial 61 ):3781. doi: 10.1016/S0378-1097 ( 03 ) 00446-4 due to a higher bioavailability dissolved! Transfer of lipophilic contaminants may be mimicked tank with a lower concentration of 2 mg/L imaging! Embryos and larvae to exert their bioactivity to increase total egg pro -,! 11 ):6108-17. doi: 10.1128/IAI.00887-06 dpf zebrafish embryo - application of embryonic and adult zebrafish for nanotoxicity assessment in... Sectioning deep inside live embryos by selective plane illumination Microscopy in zebrafish and! Ellett F, Wittbrodt J, Del Bene F, Lieschke GJ Spaink! 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